Journal: Redox Report : Communications in Free Radical Research

Article Title: LncRNA Fendrr: involvement in the protective role of nucleolin against H 2 O 2 -induced injury in cardiomyocytes

doi: 10.1080/13510002.2023.2168626

Figure Lengend Snippet: lncRNAs regulated by nucleolin in cardiomyocytes. (A) Heatmap profile of lncRNA microarray analysis. Green to red colors indicate low to high transcriptional levels. The lncRNAs differentially expressed between the two groups were identified through paired t-test P ≤ 0.05 and a fold change (FC) ≥ 2.5; n = 3 independent biological samples for each group. (B) The expression of 15 selected lncRNAs in chip test, where 10 differentially expressed up-regulated lncRNAs (up) and 5 differentially expressed down-regulated lncRNAs (down) were selected. (C) The interactions between nucleolin and lncRNAs were confirmed by RIP and identified via qRT-PCR. *, P < 0.05, vs. IgG group, n = 3. pcDNA3.1, the empty vector served as negative control; pcDNA3.1-Nuc, overexpression nucleolin group. (D) Bioinformatics website predicted Fendrr binding elements with nucleolin.

Article Snippet: Using the Aglient Rat lncRNA microarray V2.0 (Arraystar, Rockville, MD, USA), three sample pairs were prepared for lncRNA microarray analysis in the nucleolin-overexpressing rat cardiomyocyte cell line and control group.

Techniques: Microarray, Expressing, Quantitative RT-PCR, Plasmid Preparation, Negative Control, Over Expression, Binding Assay

Journal: The Journal of Biological Chemistry

Article Title: Interleukin-1β Processing Is Dependent on a Calcium-mediated Interaction with Calmodulin *

doi: 10.1074/jbc.M115.680694

Figure Lengend Snippet: Identification of pro-IL-1β interacting proteins on a human proteome microarray. A human proteome microarray was incubated with human recombinant pro-IL-1β (+) or with reagent diluent alone as a negative control (−) and probed with a mouse anti-human IL-1β antibody. An SNR value was generated for each probe set, with an SNR of >3 considered to be a hit. A comprehensive list of all identified pro-IL-1β interacting proteins (hits) is shown, including one representative negative result (for brain protein 44-like) and a positive control (anti-biotin mouse monoclonal IgG). The identity of each protein, the corresponding images for each duplicate set of proteins on the array, and the mean SNR for the duplicate protein spots are shown.

Article Snippet: Two HuProt human protein microarray slides (v.2.0) containing 19,951 probe sets spotted in duplicate were purchased from CDI Laboratories (Mayaguez, PR).

Techniques: Microarray, Incubation, Recombinant, Negative Control, Generated, Positive Control

Journal: Frontiers in Pharmacology

Article Title: Drug Repositioning for Noonan and LEOPARD Syndromes by Integrating Transcriptomics With a Structure-Based Approach

doi: 10.3389/fphar.2020.00927

Figure Lengend Snippet: Transcriptomic data information for Noonan syndrome, LEOPARD syndrome, and hypertrophic cardiomyopathy (HCM).

Article Snippet: GSE68316 , Human myocardial tissues , CapitalBio Human LncRNA Microarray v2.0 , • 7 patient samples: 7 HCM patients • 5 Control samples: 5 disease-free individuals.

Techniques: Expressing, Mutagenesis, Control, Microarray